张源

姓   名：张源

职称职务：副研究员

电子邮箱：zhangyuan@wh.iov.cn

研究方向

1.病毒的荧光标记及入侵过程动态可视化研究。

2.细胞内新型生物传感器的建立与运用。

3.病毒感染与宿主天然免疫。

教育及工作经历

2005.09-2009.07，武汉大学，生命科学学院，生物学基地班，学士学位

2009.09-2014.07，中国科学院武汉病毒研究所，博士学位

2014.07-2017.09，中国科学院武汉病毒研究所，助理研究员

2017.09-至今，中国科学院武汉病毒研究所，副研究员

近五年承担项目

1.国家自然科学基金面上项目，寨卡病毒感染人神经前体细胞（NPCs）的入侵机制及动态化研究，在研，主持。

2.国家自然科学基金青年基金，柯萨奇病毒B3型入侵神经细胞的动态过程及机制研究，完结，主持。

3.中国科学院计划-知识创新工程方向性项目，中科院青年创新促进会专项，完结，主持。

4.国家自然科学基金面上项目，Vimentin在柯萨奇病毒B3持续性感染神经细胞中的作用，在研，参与。

5.国家自然科学基金面上项目，干扰素刺激蛋白Viperin在EV71神经组织感染中所起作用及其机制，在研，参与。

6.国家自然科学基金面上项目，一种新颖的基因缺陷型EV71活疫苗的研究，完结，参与。

近五年代表性论文

1. Zhang, Y., Ke, X. L., Zheng, Z. H., Zhang, C. L., Zhang, Z. F., Zhang, F. X., Hu, Q. X., He, Z. K., and Wang, H. Z. (2013) Encapsulating Quantum Dots into Enveloped Virus in Living Cells for Tracking Virus Infection, Acs Nano 7, 3896-3904.

2. Ke, X. L., Zhang, Y., Zheng, F. L., Liu, Y., Zheng, Z. H., Xu, Y., and Wang, H. Z. (2018) SpyCatcher-SpyTag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells, Chem Commun 54, 1189-1192.(共通讯作者，共2个通讯作者)

3. Zhang, Y., Ke, X. L., Zheng, C. S., Liu, Y., Xie, L., Zheng, Z. H., and Wang, H. Z. (2017) Development of a luciferase-based biosensor to assess enterovirus 71 3C protease activity in living cells, Sci Rep-Uk 7.

4. Zhang, Y., Ke, X. L., Liu, Y., Zheng, Z. H., Meng, J., and Wang, H. Z. (2017) Fast and sensitive quantification of protein SUMOylation using a dual luciferase biosensor, Analyst 142, 608-612.

5. Zhang, C. L., Ji, X. H., Zhang, Y., Zhou, G. H., Ke, X. L., Wang, H. Z., Tinnefeld, P., and He, Z. K. (2013) One-Pot Synthesized Aptamer-Functionalized CdTe:Zn2+ Quantum Dots for Tumor-Targeted Fluorescence Imaging in Vitro and in Vivo, Anal Chem 85, 5843-5849.

6. Ke, X. L., Zhang, Y., Liu, Y., and Wang, H. Z. (2015) Identification of AcMNPV GP64-binding proteins through a combinational use of a self-biotinylated virus and the cross-linking method, Biochemical and biophysical research communications 467, 760-765.

7. Zheng, C. S., Zheng, Z. H., Sun, J. H., Zhang, Y., Wei, C. Y., Ke, X. L., Liu, Y., Deng, L., and Wang, H. Z. (2017) MiR-16-5p mediates a positive feedback loop in EV71-induced apoptosis and suppresses virus replication, Sci Rep-Uk 7.

8. Liu, Y., Tan, Z. Y., Shu, B., Zhang, Y., Zheng, C. S., Ke, X. L., Chen, X., Wang, H. Z., and Zheng, Z. H. (2017) An improved method for identifying SUMOylation sites of viral proteins, Virologica Sinica 32, 537-540.

9. Liu, Y., Zheng, Z. H., Shu, B., Meng, J., Zhang, Y., Zheng, C. S., Ke, X. L., Gong, P., Hu, Q. X., and Wang, H. Z. (2016) SUMO Modification Stabilizes Enterovirus 71 Polymerase 3D To Facilitate Viral Replication, J Virol 90, 10472-10485.

10. Xie, L., Lu, B. J., Zheng, Z. H., Miao, Y. J., Liu, Y., Zhang, Y., Zheng, C. S., Ke, X. L., Hu, Q. X., and Wang, H. Z. (2018) The 3C protease of enterovirus A71 counteracts the activity of host zinc-finger antiviral protein (ZAP), J Gen Virol 99, 73-85.

11. Li, P., Ke, X., Wang, T., Tan, Z., Luo, D., Miao, Y., Sun, J., Zhang, Y., Liu, Y., Hu, Q., Xu, F., Wang, H., and Zheng, Z. (2018) Zika Virus Attenuation by Codon Pair Deoptimization Induces Sterilizing Immunity in Mouse Models, J Virol.

12. Zhang, F. X., Zheng, Z. H., Liu, S. L., Lu, W., Zhang, Z. F., Zhang, C. L., Zhou, P., Zhang, Y., Long, G., He, Z. K., Pang, D. W., Hu, Q. X., and Wang, H. Z. (2013) Self-biotinylation and site-specific double labeling of baculovirus using quantum dots for single-virus in-situ tracking, Biomaterials 34, 7506-7518.