单超

姓名：单超

职称职务：研究员，学科组长

电子邮箱：shanchao@wh.iov.cn

研究方向

以新发传染性病毒为研究重点，主要侧重于虫媒病毒的研究，涉及以下研究方向

1.新发传染性病原动物模型建立和疫苗研发

2.虫媒病毒致病机理的探索

3.虫媒病毒病原的检测方法的建立

教育及工作经历

青岛农业大学（2003-2007），生物技术，理学学士

福建农林大学 （2007-2010），微生物学，理学硕士

中国科学院武汉病毒研究所（2010-2014），生物化学与分子生物学，理学博士

诺华热带疾病研究所（新加坡），科研助理，2013.03-2015.10

德克萨斯大学医学部（美国），博士后，2015.12-2019.12

中国科学院武汉病毒研究所，研究员，2019.12-至今

中国科学院武汉国家生物安全实验室，副主任，2022.04-至今

近五年承担项目

1. 国家海外高层次人才计划启动资金（主持）

2. 国家重点研发计划-2019-nCoV灭活疫苗（2020YFC0842100，项目骨干/病毒所负责人）

3. 国家重点研发计划-新型冠状病毒（2019-nCoV）动物模型研究（2020YFC0842000，参与）

4. 中国科学院科技服务网络计划，课题负责人

近五年代表性论文

1. Liqiang Feng*, Qian Wang*, Chao Shan*,   Chenchen Yang, Ying Feng, Jia Wu, Xiaolin Liu, Yiwu Zhou, Rendi Jiang, Peiyu   Hu, Xinglong Liu, Fan Zhang, Pingchao Li, Xuefeng Niu, Yichu Liu, Xuehua   Zheng, Jia Luo, Jing Sun, Yingying Gu, Bo Liu, Yongcun Xu, Chufang Li, Weiqi   Pan, Jincun Zhao, Changwen Ke, Xinwen Chen, Tao Xu, Nanshan Zhong, Suhua   Guan#, Zhiming Yuan#, Ling Chen#. An adenovirus-vectored COVID-19 vaccine   confers protection from SARS-COV-2 challenge in rhesus macaques.  Nat. Commun. 2020 11 (1), 1-11.   (*Co-first-authors).

2. Chao Shan#*,Yan-Feng Yao*,Xing-Lou Yang*,   Yi-Wu Zhou*,Ge Gao,Yun Peng,Lian Yang,Xue Hu,Jin Xiong,Ren-Di Jiang,   Hua-Jun Zhang,Xiao-Xiao Gao,Cheng Peng,Juan Min,Ying Chen,Hao-Rui Si,   Jia Wu,Peng Zhou,Yan-Yi Wang,Hong-Ping Wei,Wei Pang,Zheng-Fei Hu,   Long-Bao Lv,Yong-Tang Zheng,Zheng-Li Shi#,Zhi-Ming Yuan#. Infection with   Novel Coronavirus (SARS-CoV-2) Causes Pneumonia in the Rhesus Macaques. Cell   Research. (*Co-first and corresponding author).

3. Rui Shi*, Chao Shan*, Xiaomin Duan*, Zhihai   Chen*, Peipei Liu*,Jinwen Song*, Tao Song, Xiaoshan Bi, Chao Han, LianaoWu,   Ge Gao, XueHu, Yanan Zhang, Zhou Tong, WeijinHuang, William Jun Liu,   GuizhenWu, Bo Zhang, Lan Wang,Jianxun Qi, HuiFeng, Fu-Sheng Wang#,Qihui   Wang#,George Fu Gao#, Zhiming Yuan#, Jinghua Yan#. A Human Neutralizing   Antibody Targets the Receptor Binding Site of SARS-CoV-2. Nature 2020 May 26.   doi: 10.1038/s41586-020-2381-y. (*Co-first-authors, # Co-corresponding   author).

4. Chao Shan*,Hongjie Xia*,Sherry L Haller,   Sasha R Azar,Yang Liu,Jianying Liu,Antonio E Muruato,Rubing Chen,Shannan   L Rossi,Maki Wakamiya,Nikos Vasilakis,Rongjuan Pei,Camila R   Fontes-Garfias,Sanjay Kumar Singh,Xuping Xie,Scott C Weaver#,Pei-Yong   Shi#. A Zika virus envelope mutation preceding the 2015 epidemic enhances   virulence and fitness for transmission. PNAS,2020,117 (33):20190-20197.   (*Co-first-authors,# Co-corresponding author).

5. Maria I Giraldo*, HongjieXia*, Leopoldo   Aguilera-Aguirre, Adam Hage, Sarah vanTol, Chao Shan, Xuping Xie, GailL   Sturdevant, Shelly J Robertson, Kristin L McNally, KimberlyMeade-White,   Sasha R Azar, Shannan L Rossi,Wendy Maury,Michael Woodson,Holly Ramage,   Jeffrey R Johnson, Nevan J Krogan, Marc C Morais, Sonja M Best, Pei-Yong   Shi#, Ricardo Rajsbaum#. Envelope proteinubiquitinationdrives entry and pathogenesis   ofZika virus. Nature, 2020, 585 (7825): 414-419. (*Co-first-authors, #   Co-corresponding author).

6. Ren-Di Jiang*,Mei-Qin Liu*,Ying Chen*,   Chao Shan,Yi-Wu Zhou,Xu-Rui Shen,Qian Li,Lei Zhang,Yan Zhu,Hao-Rui Si,   Qi Wang,Juan Min,Xi Wang,Wei Zhang,Bei Li,Hua-Jun Zhang,Ralph S Baric,   Peng Zhou,Xing-Lou Yang#,Zheng-Li Shi#. Pathogenesis of SARS-CoV-2 in   transgenic mice expressing human angiotensin-converting enzyme 2. Cell,2020,   182 (1):50-58. (*Co-first-authors,# Co-corresponding author).

7. Pei Xu,Chao Shan,Tiffany J Dunn,Xuping   Xie,Hongjie Xia,Junling Gao,Javier Allende Labastida,Jing Zou,Paula P   Villarreal,Caitlin R Schlagal,Yongjia Yu,Gracie Vargas,Shannan L Rossi,   Nikolaos Vasilakis,Pei-Yong Shi,Scott C Weaver#,Ping Wu#. Role of   microglia in the dissemination of Zika virus from mother to fetal brain. PLoS   neglected tropical diseases,2020,14 (7):e0008413. (# Co-corresponding   author). 

8. Huanle Luo,Guangyu Li,Binbin Wang,Bing   Tian,Junling Gao,Jing Zou,Shuizhen Shi,Shuang Zhu,Bi-Hung Peng,   Awadalkareem Adam,Ariza Martinez,Kimberly Hein,Evandro R Winkelmann,   Yoseph Mahmoud,Xiaofei Zhou,Chao Shan,Shannan Rossi,Scott Weaver,Alan DT   Barrett,Shao-Cong Sun,Wenbo Zhang,Pei-Yong Shi,Ping Wu,Tian Wang. Peli1   signaling blockade attenuates congenital zika syndrome. PLoS pathogens,2020,   16 (6):e1008538.

9. Bruno TD Nunes*,Camila R Fontes-Garfias*,   Chao Shan,Antonio E Muruato,Jannyce GC Nunes,Rommel MR Burbano,Pedro FC   Vasconcelos,Pei-Yong Shi#,Daniele BA Medeiros#.  Zika structural genes determine the   virulence of African and Asian lineages. Emerging Microbes & Infections,   2020,1-27.

10. Anzhong Li,Mijia Lu,Jingyou Yu,Olivia   Harder,Zayed Attia,Chao Shan,Xueya Liang,Miaoge Xue,Pei-Yong Shi,Mark E   Peeples,Shan-Lu Liu,Prosper N Boyaka,Stefan Niewiesk,Jianrong Li. Novel   non-segmented negative-sense RNA virus-based vaccine platforms for Zika   virus. The Journal of Immunology,2020,204 (1):166.10-166.10.

11. Chao Shan*,Xuping Xie*,Huanle Luo,Antonio   E Muruato,Yang Liu,Maki Wakamiya,Jun-Ho La,Jin Mo Chung,Scott C Weaver,   Tian Wang,Pei-Yong Shi. Maternal vaccination and protective immunity against   Zika virus vertical transmission. Nat. Commun. 2019;10(1):1-12. (*Co-first-authors).

12. Antonio E Muruato*,Chao Shan*,Camila R   Fontes-Garfias,Yang Liu,Zengguo Cao,Qiang Gao,Scott C Weaver,Pei-Yong   Shi. Genetic stability of live-attenuated Zika vaccine candidates. Antiviral   research. 2019,171: 104596.    (*Co-first-authors).

13. Guangyu Li,Awadalkareem Adam,Huanle Luo,   Chao Shan,Zengguo Cao,Camila R Fontes-Garfias,Vanessa V Sarathy,Cody   Teleki,Evandro R Winkelmann,Yuejin Liang,Jiaren Sun,Nigel Bourne,Alan DT   Barrett,Pei-Yong Shi,Tian Wang. An attenuated Zika virus NS4B protein   mutant is a potent inducer of antiviral immune responses. npj Vaccines. 2019;   4 (1):1-10.

14. A single-dose plasmid-launched   live-attenuated Zika vaccine induces protective immunity.Jing Zou,Xuping   Xie,Huanle Luo,Chao Shan,Antonio E. Muruato,Scott C Weaver,Tian Wang,   Pei-Yong Shi. EBioMedicine. 2018 Sep 7. pii: S2352-3964(18)30349-9.

15. Using a virion assembly-defective dengue   virus as a vaccine approach. Chao Shan*,Xuping Xie*,Jing Zou,Roland Züst,   Bo Zhang,Rebecca Ambrose,Jason Mackenzie,Katja Fink,Pei-Yong Shi. J   Virol. 2018 Aug 15. pii: JVI.01002-18. (*Co-first authors).SPOTLIGHT   article.

16. Zika Virus Vaccine: Progress and Challenges;   Chao Shan,Xuping Xie,Pei-Yong Shi. Cell Host and Microbe;2018,   https://doi.org/10.1016/j.chom.2018.05.021.

17. Did Zika Virus Mutate to Cause Severe   Outbreaks?Shannan L Rossi,Gregory D Ebel,Chao Shan,Pei-Yong Shi,Nikos   Vasilakis. Trends Microbiol. 2018 Jun 11. pii: S0966-842X(18)30111-2.

18. Human IFIT3 modulates IFIT1 RNA binding specificity   and protein stability. Britney Johnson,Laura A VanBlargan,Wei Xu,James P   White,Chao Shan,Pei-Yong Shi,Rong Zhang,Jagat Adhikari,Michael L Gross,   Daisy W Leung,Michael S Diamond,Gaya K Amarasinghe. Immunity. 2018,48(3):   487-499. e5.

19. Potential Mechanisms for Enhanced Zika   Epidemic and Disease. Hongjie Xia,Xuping Xie,Chao Shan#,and Pei-Yong Shi#.   ACS Infect Dis. 2018,4 (5),pp 656–659. (Co-corresponding author)

20. An evolutionary NS1 mutation enhances Zika   virus evasion of host interferon induction. Hongjie Xia,Huanle Luo,Chao   Shan,Antonio E. Muruato,Bruno T.D. Nunes,Daniele B.A. Medeiros,Jing Zou,   Xuping Xie,Maria Isabel Giraldo,Pedro F.C. Vasconcelos,Scott C. Weaver,   Tian Wang,Ricardo Rajsbaum,Pei-Yong Shi. Nat. Commun. 2018;9(1):414.

21. Zika virus infection elicits auto-antibodies   to C1q. Takaaki Koma,Veljko Veljkovic,Danielle E Anderson,Lin-Fa Wang,   Shannan L Rossi,Chao Shan,Pei-Yong Shi,David W Beasley,Natalya Bukreyeva,   Jeanon N Smith,Steven Hallam,Cheng Huang,Veronika von Messling,Slobodan   Paessler. Scientific reports. 2018,8(1):1882.

22. Characterization of a candidate tetravalent   vaccine based on 2'-O-methyltransferase mutants. Roland Züst,Shi-Hua Li,   Xuping Xie,Sumathy Velumani,Melissa Chng,Ying-Xiu Toh,Jing Zou,Hongping   Dong,Chao Shan,Jassia Pang,Cheng-Feng Qin,Evan W Newell,Pei-Yong Shi,   Katja Fink. PloS one. 2018,13(1):e0189262.

23. Xuping Xie,Jing Zou,Chao Shan,Pei-Yong   Shi. Small Molecules and Antibodies for Zika Therapy. J Infect Dis. 2017;216   (suppl_10):S945-S950.

24. Camila R. Fontes-Garfias*,Chao Shan*,Huanle   Luo,Antonio E. Muruato,Daniele B.A. Medeiros,Elizabeth Mays,Xuping Xie,   Jing Zou,Christopher M. Roundy,Maki Wakamiya,Shannan L. Rossi,Tian Wang,   Scott C. Weaver,Pei-Yong Shi. Functional Analysis of Glycosylation of Zika   Virus Envelope Protein. Cell Rep. 2017;21(5):1180-1190. (*Co-first   authors).

25. Chao Shan,Antonio E. Muruato,Brett W.   Jagger,Justin Richner,Bruno T.D. Nunes,Daniele B.A. Medeiros,Xuping Xie,   Jannyce G.C. Nunes,Kaitlyn M. Morabito,Wing-Pui Kong,Theodore C. Pierson,   Alan D. Barret,Scott C. Weaver,Shannan L. Rossi,Pedro F.C. Vasconcelos,   Barney S. Graham*,Michael S. Diamond*,Pei-Yong Shi*. A single-dose   live-attenuated vaccine prevents Zika virus pregnancy transmission and testis   damage. Nat. Commun. 2017;8(1):676.

26. Zhe Zhu,Matthew J. Gorman,Lisa D. McKenzie,   Jiani N. Chai,Christopher G. Hubert,Briana C. Prager,Estefania Fernandez,   Justin M. Richner,Rong Zhang,Chao Shan,Xiuxing Wang,Pei-Yong Shi,Michael   S. Diamond,Jeremy N. Rich,Milan G. Chheda. Zika virus has oncolytic   activity against glioblastoma stem cells. J Exp Med. 2017;214(10):   2843-2857.

27. Julien Pompon,Menchie Manuel,Geok Kee Ng,   Benjamin Wong,Chao Shan,Gayathri Manokaran,Ruben Soto-Acosta,Shelton S.   Bradrick,Eng Eong Ooi,Dorothée Missé,Pei-Yong Shi,Mariano A.   Garcia-Blanco. Dengue subgenomic flaviviral RNA disrupts immunity in mosquito   salivary glands to increase virus transmission. PLoS Pathog. 2017;13(7):   e1006535.

28. Chao Shan*,Daniel A. Ortiz*,Yujiao Yang,   Susan J. Wong,Laura D. Kramer,Pei-Yong Shi,Michael J. Loeffelholz,Ping   Ren. Evaluation of a Novel Reporter Virus Neutralization Test for the   Serological Diagnosis of Zika and Dengue Virus Infection. J Clin Microbiol.   2017;55(10):3028-3036. (*Co-first-authors).

29. Justin M. Richner*,Brett W. Jagger*,Chao   Shan*,Camila R. Fontes*,Kimberly A. Dowd*,Bin Cao,Sunny Himansu,   Elizabeth A. Caine,Bruno T.D. Nunes,Daniele B.A. Medeiros,Antonio E.   Muruato,Bryant M. Foreman,Huanle Luo,Tian Wang,Alan D. Barrett,Scott C.   Weaver,Pedro F.C. Vasconcelos,Shannan L. Rossi,Giuseppe Ciaramella,Indira   U. Mysorekar,Theodore C. Pierson,Pei-Yong Shi,Michael S. Diamond. Vaccine   Mediated Protection Against Zika Virus-Induced Congenital Disease. Cell 2017;   170(2):273-283.e12. (*Co-first-authors).

30. Yang Liu*,Jianying Liu*,Senyan Du*,Chao   Shan*,Kaixiao Nie,Xiaofeng Li,Tao Wang,Cheng-Feng Qin,Penghua Wang,   Pei-Yong Shi,Gong Cheng. Evolutionary enhancement of Zika virus infectivity   in mosquitos. Nature 2017;545(7655):482-486. (*Co-first-authors).

31. Chao Shan,Antonio E. Muruato,Bruno T.D.   Nunes,Huanle Luo,Xuping Xie,Daniele B.A. Medeiros,Robert B. Tesh,Alan D.   Barrett,Tian Wang,Scott C. Weaver,Pedro F.C. Vasconcelos,Shannan L.   Rossi,Pei-Yong Shi. A live-attenuated Zika virus vaccine induces sterilizing   immunity in mouse models. Nat Med. 2017;23(6):763-767.

32. Chao Shan,Xuping Xie,Ping Ren,Michael J.   Loeffelholz,Yujiao Yang,Andrea Furuya,Alan P. Dupuis II,Laura D. Kramer,   Susan J. Wong,and Pei-Yong Shi,A rapid Zika diagnostic assay to measure   neutralizing antibodies in patients. EBioMedicine 2017;17:157-162.

33. Yujiao Yang*,Chao Shan*,Jing Zou,Antonio   E. Muruato,Diniz Nunes Bruno,Barbosade Almeida Medeiros Daniele,Pedro F.C.   Vasconcelos,Shannan L.Rossi,Scott C. Weaver,Xuping Xie,Pei-Yong Shi. A   cDNA Clone-Launched Platform for High-Yield Production of Inactivated Zika   Vaccine. EBioMedicine 2017;17: 145-156. (*Co-first-authors).

34. Xuping Xie,Jing Zou,Chao Shan,Yujiao Yang,   Dieudonné Buh Kum,Kai Dallmeier,Johan Neyts,Pei-Yong Shi. Zika Virus   Replicons for Drug Discovery. EBioMedicine 2016 (12):156–160.

35. Chao Shan,Xuping Xie,Antonio E. Muruato,   Shannan L. Rossi,Christopher M. Roundy,Sasha R. Azar,Yujiao Yang,Robert   B. Tesh,Nigel Bourne,Alan D. Barrett,Nikos Vasilakis,Scott C. Weaver,and   Pei-Yong Shi,An Infectious cDNA Clone of Zika Virus to Study Viral   Virulence,Mosquito Transmission,and Antiviral Inhibitors. Cell Host Microbe   2016;19(6):891-900.

36. Xuping Xie,Chao Shan,Pei-Yong Shi.   Restriction of Zika Virus by Host Innate Immunity. Cell Host Microbe 2016;19(5):   566-7.

37. Chao Shan,Xuping Xie,Alan D. T. Barrett,   Mariano A. Garcia-Blanco,Robert B. Tesh,Pedro Fernando da Costa   Vasconcelos,Nikos Vasilakis,Scott C. Weaver,and Pei-Yong Shi. Zika Virus:   Diagnosis,Therapeutics,and Vaccine. ACS Infect Dis. 2016;2(3):170-2.

38. Pantao Zhang,Chao Shan,Xiaodan Li,Siqing   Liu,Chenglin Deng,Hanqing Ye,Baodi Shang,Pei-Yong Shi,Ming Lv,Beifen   Shen,Chengfeng Qin,Bo Zhang. Generation of a recombinant West Nile virus   stably expressing the Gaussia luciferase for neutralization assay. Virus Res.   2016;211: 17-24.

39. Linlin Xu,Chao Shan,Chenglin Deng,Xiaodan   Li,Baodi Shang,Hanqing Ye,Siqing Lu,Zhiming Yuan,Qingyin Wang,Pei-Yong   Shi,Bo Zhang. Development of a stable Gaussia luciferase enterovirus 71   reporter virus. J Virol Methods. 2015;219: 62-6.

40. Xiao-Dan Li,Chao Shan,Cheng-Lin Deng,   Han-Qing Ye,Pei-Yong Shi,Zhi-Ming Yuan,Peng Gong,Bo Zhang. The Interface   between Methyltransferase and Polymerase of NS5 is Essential for Flavivirus   Replication. PLoS Negl Trop Dis. 2014;8(5):e2891.

41. Cheng Chen*,Yaxin Wang*,Chao Shan*,Yuna   Sun,Peng Xu,Honggang Zhou,Cheng Yang,Pei-Yong Shi,Zihe Rao,Bo Zhang#,   Zhiyong Lou#. Crystal structure of enterovirus 71 RNA-dependent RNA   polymerase complexed with its protein primer VPg: implication for a trans   mechanism of VPg uridylylation. J Virol. 2013,87 (10):5755-5768.   (*Co-first-authors).

42. Chao Shan,Xiaodan Li,Chenglin Deng,Baodi   Shang,Linlin Xu,Hanqing Ye,Zhiming Yuan and Bo Zhang. Development and   Characterization of West Nile Virus Replicon Expressing Secreted Gaussia   Luciferase. Virol Sin. 2013,28 (3):161-166.

43. Yuna Sun*,Yaxin Wang*,Chao Shan*,Cheng   Chen,Peng Xu,Mohan Song,Honggang Zhou,Cheng Yang,Wenbo Xu,Pei-Yong Shi,   Bo Zhang#,Zhiyong Lou#. Entrovirus-71 VPg uridylation uses a two-molecular   mechanism of 3Dpol. J Virol. 2012,86(24):13662-13671 (*Co-first-authors).

专利：

Reverse genetic   system of Zika virus,Pei-Yong Shi,Chao Shan,Xuping Xie;2020/01/14,US   Patent 10,533,997.

CDNA clone-launched   platform for high-yield production of inactivated zika virus,Xuping Xie,   Chao Shan,Pei-Yong Shi;2019/3/26,US patent number: 10,240,130.