罗敏华

姓       名：罗敏华

职称职务：研究员

电子邮箱：luomh@wh.iov.cn

研究方向

1.解析嗜神经疱疹病毒（人巨细胞病毒HCMV、单纯疱疹病毒HSV-1、水痘-带状疱疹病毒VZV）致神经损伤机制

2.改造病毒，解析病毒的结构和功能；发展和优化神经环路示踪工具，揭示神经回路结构和功能；研发新型疫苗

教育及工作经历

1983.09-1988.07  湖南医科大学，临床医学学士

1992.09-1995.07  湖南医科大学，医学微生物学，硕士

1996.09-1999.07  湖南医科大学，内科/传染病学，博士

1988.07-2000.11  中南大学（湖南医科大学），医学微生物教研室，助教-副教授

2000.04-2002.08   Harvard Medical School，E.K. Shriver Center，博士后

2002.08-2004.11  中南大学，湘雅医学院，教授

2004.12-2007.12   University of Idaho，Microbiology，Research Scientist

2008.01-2010.10   University of Idaho，Microbiology，Assistant Professor

2009.07-至今      中科院武汉病毒研究所，研究员

2014.01-至今      中科院脑科学和智能技术卓越中心，特聘研究员

2015.04-至今      中国科学院大学岗位教授（A类）

2017.07-至今      中科院武汉病毒研究所，研究员(二级、特聘）

近五年承担项目

1.  新型神经减毒疫苗的研发，国药集团，50886万，2020-2025，主持。

2.  Project Title: Genetically engineered anterograde monosynaptic viral tracers for multi-species neural circuit analysis,NIH (1RF1MH120020-01), $75.8万，2019-2022，CoPI.

3.   脑研究新技术研发-神经环路示踪.中国科学院战略性先导科技专项（B类, XDB32000000）, 400万，2018-2022，骨干。

4.   先天性巨细胞病毒感染下调Sox2影响胎脑发育的机制。国家自然科学基金国际合作重点项目(81620108021)，240万，2017-2021，结题，优秀。

5.   老年痴呆记忆损伤的神经环路机制和干预策略,国家自然科学基金情感与记忆环路重大研究计划集成项目（91632306），90/240万, 2017-2019,结题。

6.   基于荧光分子纳米分辨定位的超分辨定位成像仪器。家自然科学基金重大科研仪器研制项目（81427801），250/750万，2015-2019，结题。

7.   灵长类神经回路精细结构成像的新方法和新工具。科技部973（2015CBR55600），157万，2015-2019，结题。

8.   新型神经环路示踪病毒工具的研制。中国科学院前沿科学重大突破，115万/年，2014-2019，结题。

近五年代表性论文

通讯作者论文：

1.Xue-Hui Ma#, Yongxuan Yao#, Xian-Zhang Wang, Yue-Peng Zhou，Sheng-Nan Huang，Dong Li, Meng-Jie Mei，Jing-Peng Wu, Yu-Ting Pan,Shuang Cheng, Jin-Yan Sun, Wen-Bo Zeng, Sitang Gong, Han Cheng* , Min-Hua Luo*, Bo Yang*. MORC3 Restricts Human Cytomegalovirus Infection by Suppressing the Major Immediate-early Promoter Activity. J Med Virol (IF=18.6) 2022.

2.Feng Xiong#, Hong Yang#, Yi-Ge Song, Hai-Bin Qin, Qing-Yang Zhang, Xian Huang, Wei Jing, Manfei Deng, Yang Liu, Zhixiang Liu, Yin Shen, Yunyun Han, Youming Lu, Xiangmin Xu, Todd Holmes, Minmin Luo, Fei Zhao*, Min-Hua Luo*, Wen-Bo Zeng*. A novel HSV-1-H129 amplicon tracer system allows for rapid and efficient monosynaptic anterograde neural circuit tracing. Nat. Comms (revising).

3.Zhou YP, Mei MJ, Wang XZ, Huang SN, Chen L, Zhang M, Li XY, Qin HB, Dong X, Cheng S, Wen L,Yang B, An XF, He AL, Zhang B, Zeng WB,  Li XJ, Lu Y, Li HC, Li HD, Zou WG, Alec J. Redwood AJ,  Rayner S, Cheng H, McVoy MA, Tang Q, Britt WJ*, Zhou X*, Jiang X*, Luo MH*. A congenital CMV infection model for follow-up studies of neurodevelopmental disorders, neuroimaging abnormalities, and treatment. JCI Insight. 2022 Jan 11;7(1):e152551. doi: 10.1172/jci.insight.152551.（highlighted research）

4.Yang H; Xiong F; Qin HB; Yu QT; Sun JY; Zhao HW; Li D; Zhou Y; Zhang FK; Zhu XW; Wu T; Jiang M; Xu X; Lu Y; Shen HJ; Zeng WB*; Zhao F*, Luo MH*. A novel H129-based anterograde monosynaptic tracer exhibits features of strong labeling intensity, high tracing efficiency, and reduced retrograde labeling. Mol Neurodeger. 2022 Jan 10;17(1):6. doi: 10.1186/s13024-021-00508-6.

5.Yang B, Yao Y, Cheng H, Wang XZ, Zhou YP, Huang SN, Ma X, Yang H, Wu J, Jiang X, Cheng S, Sun JY, Zeng WB, Chen J, Zhang FK, Shen HJ, Gu JY, McVoy MA, Britt WJ, Gong S, Luo MH.Human Cytomegalovirus Hijacks WD Repeat Domain 11 for Virion Assembly Compartment Formation and Virion Morphogenesis.J Virol. 2022 Jan 12:JVI0182721. doi: 10.1128/JVI.01827-21.

6.Cheng S, Zhao F, Wen L, Yang B, Wang XZ, Huang SN, Jiang X, Zeng WB, Sun JY, Zhang FK, Shen HJ, Fortunato E, Luo MH* Cheng H*. iTRAQ-based proteomics analysis of HCMV latency and reactivation in T98G cells. J Virol. 2022 Jan 26;96(2):e0147621. doi: 10.1128/JVI.01476-21. Epub 2021 Nov 3.PMID: 34730396.

7.Yang B, Yao Y, Wu H, Yang H, Ma XH, Li D, Wang XZ, Huang SN, Jiang X, Cheng S, Sun JY, Huang ZL, Zhao C, McVoy MA, Ahn JH, Zeng WB, Britt WJ, Gong S, Luo MH*.  Localization of the WD repeat-containing protein 5 to the Virion Assembly Compartment Facilitates Human Cytomegalovirus Assembly.J Virol. 2021 doi: 10.1128/JVI.02101-20. Online ahead of print.

8.Huang SN, Zhou YP, Jiang X, Yang B, Cheng H*, Luo MH*. Hearing Loss Caused by HCMV Infection through Regulating the Wnt and Notch Signaling Pathways. Viruses. 2021 Apr 6;13(4):623. doi: 10.3390/v1304062.

9.YangH, XiongF, Song YG, JiangHF, QinHB,ZhouJ, LuS, GriecoSF, XuX, ZengWB*, ZhaoF*, LuoMH*.  HSV-1 H129-Derived Anterograde Neural Circuit Tracers: Improvements, Production, and Applications. Neurosci Bull. 2020 doi: 10.1007/s12264-020-00614-3.

10.Li D, Yang H, Xiong F, Xu X, Zeng WB, Zhao F*, Luo MH*. Anterograde Neuronal Circuit Tracers Derived from Herpes Simplex Virus 1: Development, Application, and Perspectives.Int J Mol Sci. 2020 Aug 18;21(16):5937. doi: 10.3390/ijms21165937.

11.Sun J, Liu C, Peng R, Zhang FK, Tong Z, Liu S, Shi Y, Zhao Z, Zeng WB, Gao GF, Shen HJ, Yang X*, Luo M*, Qi J*, Wang P*. Cryo-EM structure of the varicella-zoster virus A-capsid. Nat Commun. 2020 Sep 22;11(1):4795. doi: 10.1038/s41467-020-18537-y.

12.Dong X, Zhou J, Qin HB, Xin B, Huang ZL, Li YY, Xu XM, Zhao F, Zhao CJ*, Liu JJ*, Luo MH*, Zeng WB*. Anterograde Viral Tracer Herpes Simplex Virus 1 Strain H129 Transports Primarily as Capsids in Cortical Neuron Axons. J Virol. 2020; 94(8):e01957-19

13.Wen L, Zhao F, Qiu Y, Cheng S, Sun JY, Fang W, Rayner S, McVoy MA, Jiang XJ, Tang Q, Li FC*, Hu F*, Luo MH*. Human Cytomegalovirus DNA and Immediate Early Protein 1/2 Are Highly Associated With Glioma and Prognosis. Protein Cell. 2020 Mar 18. doi: 10.1007/s13238-020-00696-9

14.Zeng WB, Zhang F, Cheng S, Sun JY, Shen H, Luo MH*. Concerns on Vaccine Against Varicella Caused by Varicella-Zoster Virus Infection. Virol Sin. 2020 May 28. doi: 10.1007/s12250-020-00231-4

15.Wu CC, Jiang X, Wang XZ, Liu XJ, Li XJ, Yang B, Ye HQ, Harwardt T, Jiang M, Xia HM, Wang W, Britt WJ, Paulus C, Nevels M*, Luo MH*.Human Cytomegalovirus Immediate-Early 1 Protein Causes Loss of SOX2 from Neural Progenitor Cells by Trapping Unphosphorylated STAT3 in the Nucleus.J Virol. 2018 Jun 27. pii: JVI.00340-18

16.Jiang X, Dong X, Li SH, Zhou YP, Rayner S, Xia HM, Gao GF, Yuan H, Tang YP*, Luo MH*.Proteomic Analysis of Zika Virus Infected Primary Human Fetal Neural Progenitors Suggests a Role for Doublecortin in the Pathological Consequences of Infection in the Cortex.Front Microbiol. 2018; 9:1067

17.Yang B, Liu XJ, Yao YX, Jiang X, Wang XZ, Yang H, Sun JY, Miao Y, Wang W, Huang ZL, Wang YY, Tang Q, Rayner S, Britt WJ, McVoy MA, Luo MH*, Fei Zhao1,2* WDR5 Facilitates Human Cytomegalovirus Replication by Promoting Capsid Nuclear Egress. J Virol, 2018; 92(9) (doi: 10.1128/JVI.00207-18.

18.Liu XJ, Yang Y, Huang SN, Wu CC, Li XJ, Cheng S, Jiang X, Hu F, Ming YZ, Nevels M, Britt WJ, Rayner S, Tang Q, Zeng WB*, Zhao Z*, Luo MH*. Human Cytomegalovirus IE1 Downregulates Hes1 in Neural Progenitor Cells as a potential E3 Ubiquitin Ligase. PLoS Pathog. 2017; 13(7):e1006542.

19.Zeng WB#, Jiang HF#, Gang YD, Song YG, Shen ZZ, Yang H, Dong X, Tian YL, Ni RJ, Liu Y, Tang N, Li X, Jiang X, Gao D, Androulakis M, He XB, Xia HM, Ming YZ, Lu Y, Zhou JN, Zhang C, Xia XS, Shu Y, Zeng SQ, Xu F*, Zhao F*, Luo MH*. Anterograde monosynaptic transneuronal tracers derived from herpes simplex virus 1 strain H129. Mol Neurodegener. 2017 May 12;12(1):38. doi: 10.1186/s13024-017-0179-7.

10.Jiang HF, Wang W, Jiang X, Zeng WB, Shen ZZ, Song YG, Yang H, Liu XJ, Dong X, Zhou J, Sun JY, Yu FL, Guo L, Cheng T, Rayner S, Zhao F, Zhu H*, Luo MH*. ORF7 of Varicella-Zoster Virus Is Required for Viral Cytoplasmic Envelopment in Differentiated Neuronal Cells. J Virol. 2017; 91(12). pii: e00127-17.

21.Cheng S, Jiang X, Yang B, Wen L, Zhao F, Zeng WB, Liu XJ, Dong X, Sun JY, Ming YZ, Zhu H, Rayner S, Tang Q, Fortunato E*, Luo MH*. Infected T98G glioblastoma cells support human cytomegalovirus reactivation from latency. Virology. 2017; 510:205-215.

22.Zhao F, Jiang HF, Zeng WB, Shu Y, Luo MH*, Duan S. Anterograde Trans-Synaptic Tagging Mediated by Adeno-Associated Virus. Neurosci Bull. 2017; 33:348-350.

专利

1.PCT/CN2021/101401 Luo MH, Zeng WB, Zhao F, Yang H. ANTEROGRADE MONOSYNAPTIC TRANSNEURONAL TRACER SYSTEM WITH GK PROTEIN FUNCTION MANIPULATION (application date 6/22/2021)

2.ZL202110694878.X 罗敏华，曾文波，杨虹，熊锋，赵非.调控gK蛋白功能的顺行跨单级神经示踪系统。

3.PCT/CN2016/104882   Luo MH, Zeng WB, Jiang HF, Zhao F, Yang H, Song YG, Shen ZZ. Anterograde mono-synaptic transneuronal tracer system (国际专利，授权公司生产销售)

4.US10，494，635 B2/2019.12   Luo MH, Zeng WB, Jiang HF, Zhao F, Yang H, Song YG, Shen ZZ. Anterograde multi-synaptic transneuronal tracer (国际专利，授权公司生产销售)

5.201610976316.3罗敏华，曾文波，江海飞，赵非，杨虹，宋一舸，谌章州。顺行跨单级突触神经示踪系统。

6.ZL201610976085.6罗敏华，曾文波，江海飞，赵非，杨虹，宋一舸，谌章州。顺行跨多级突触、跨神经元的示踪系统 （2020授权，授权公司生产销售）。

7.201511029238.8罗敏华，谌章州，柳中洋，赵非，阮强，朱桦。HSV1-H129-BAC及其变体的构建方法与应

8.ZL201510287275.2罗敏华，曾文波，江海飞，赵非，谌章州。人巨细胞病毒感染性克隆及其构建方法和应用（2020授权）。

 9.ZL201010529015.9罗敏华，段荧亮，伊丽莎白福尔图纳 托。一种基于细胞怕片的快速经济的免疫荧光方法。（2013批准授权，2016年转让）通讯作者论文：