狨猴（marmoset）是研究中东呼吸综合征（Middle Eastern Respiratory Syndrome）的最佳动物模型。一直以来，由于没有找到感染病毒后与人类有相似呼吸道症状的动物，对MERS冠状病毒的研究一直没有取得突破性进展。 8月21日，美国国家过敏与传染性疾病研究所( US National Institute of Allergy and Infectious Diseases，NIAID)的研究者发布了两份研究称，狨猴能够感染MERS病毒，并出现与人类重症肺炎极为类似的症状。
Infection with MERS-CoV Causes Lethal Pneumonia in the Common Marmoset
D. Falzaranoet al.PLoS Pathog.10,e1004250 (2014) doi: 10.1371/journal.ppat.1004431
Abstract
The availability of a robust disease model is essential for the development of countermeasures for Middle East respiratory syndrome coronavirus (MERS-CoV). While a rhesus macaque model of MERS-CoV has been established, the lack of uniform, severe disease in this model complicates the analysis of countermeasure studies. Modeling of the interaction between the MERS-CoV spike glycoprotein and its receptor dipeptidyl peptidase 4 predicted comparable interaction energies in common marmosets and humans. The suitability of the marmoset as a MERS-CoV model was tested by inoculation via combined intratracheal, intranasal, oral and ocular routes. Most of the marmosets developed a progressive severe pneumonia leading to euthanasia of some animals. Extensive lesions were evident in the lungs of all animals necropsied at different time points post inoculation. Some animals were also viremic; high viral loads were detected in the lungs of all infected animals, and total RNAseq demonstrated the induction of immune and inflammatory pathways. This is the first description of a severe, partially lethal, disease model of MERS-CoV, and as such will have a major impact on the ability to assess the efficacy of vaccines and treatment strategies as well as allowing more detailed pathogenesis studies.
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《Nature》新闻一周热点-“中东呼吸综合征”找到动物模型
狨猴（marmoset）是研究中东呼吸综合征（Middle Eastern Respiratory Syndrome）的最佳动物模型。一直以来，由于没有找到感染病毒后与人类有相似呼吸道症状的动物，对MERS冠状病毒的研究一直没有取得突破性进展。 8月21日，美国国家过敏与传染性疾病研究所( US National Institute of Allergy and Infectious Diseases，NIAID)的研究者发布了两份研究称，狨猴能够感染MERS病毒，并出现与人类重症肺炎极为类似的症状。
新闻链接：http://www.nature.com/news/seven-days-22-28-august-2014-1.15767
Infection with MERS-CoV Causes Lethal Pneumonia in the Common Marmoset
D. Falzarano et al. PLoS Pathog. 10, e1004250 (2014) doi: 10.1371/journal.ppat.1004431
Abstract
The availability of a robust disease model is essential for the development of countermeasures for Middle East respiratory syndrome coronavirus (MERS-CoV). While a rhesus macaque model of MERS-CoV has been established, the lack of uniform, severe disease in this model complicates the analysis of countermeasure studies. Modeling of the interaction between the MERS-CoV spike glycoprotein and its receptor dipeptidyl peptidase 4 predicted comparable interaction energies in common marmosets and humans. The suitability of the marmoset as a MERS-CoV model was tested by inoculation via combined intratracheal, intranasal, oral and ocular routes. Most of the marmosets developed a progressive severe pneumonia leading to euthanasia of some animals. Extensive lesions were evident in the lungs of all animals necropsied at different time points post inoculation. Some animals were also viremic; high viral loads were detected in the lungs of all infected animals, and total RNAseq demonstrated the induction of immune and inflammatory pathways. This is the first description of a severe, partially lethal, disease model of MERS-CoV, and as such will have a major impact on the ability to assess the efficacy of vaccines and treatment strategies as well as allowing more detailed pathogenesis studies.
论文链接：http://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1004250